Quality Assurance of Immunophenotyping
	Summary Of Recommendations For Quality Assurance Of Peripheral Blood Immunophenotyping Review, understand and apply universal precautions in handling peripheral blood specimens For specimens that can be analyzed within 6 hours use EDTA anticaogulant Always transport and maintain specimens at room temperature (18-22 degrees C). DO NOT REFRIGERATE. To prepare specimen use staining and whole blood lysis Be conservative, use the minimum number of antibodies needed to characterize a population of cells Participate in an outside proficiency testing program Run a daily minimal positive and negative control Use the forward angle versus orthogonal gating for lymphocyte population analysis Archive and save all histograms and analysis regions used to create reported results Report cluster of differentiation results as percent positive and by absolute numbers DNA Cell Cycle Analysis Last Modified: 8/11/97, Copyright © 1994, 1995 University of Texas - Houston Medical School, DPALM MEDIC All rights reserved.